Hi Rob, best way would be to load all of your stacks and register them again. I see I forget a dropdown down box at save registered frames, I will fix this now.
When you have registered them,
go to 6) integration, set composition to full and click on apply mode.
If you then click on save registered frames, wou won’t lose data and all frames will have the same image dimensions.
I need to create the dropdownbox for composition at save registered frames.. Will do it now.. ?
Let me know it it works ?
And if it works, then proceed to combine RGB indeed ? and possibly selective color afterwards for finetuning of the colors.
The combine RGB will adjust for illumination differences to start with ? so the background of your different channels will almost be perfect to start with.